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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

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06:56 min

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March 31, 2017

DOI:

10.3791/55463-v

DOI:
10.3791/55463-v

•

06:56 min
March 31, 2017

•

3 Views
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1Department of Plant and Microbial Biology,University of California Berkeley, 2John Innes Centre,Norwich Research Park, 3Department of Plant and Microbial Biology,University of Zürich, 4USDA-ARS Sunflower and Plant Biology Research Unit

Summary

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Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.

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DNA ExtractionGenomic DNAPowdery MildewLong-read SequencingConidiaLysis BufferSarkosylChloroform Isoamyl AlcoholIsopropanolEthanolTE BufferRNase

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Feehan, J. M., Scheibel, K. E., Bourras, S., Underwood, W., Keller, B., Somerville, S. C. Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing. J. Vis. Exp. (121), e55463, doi:10.3791/55463 (2017).

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