Journal
/
Genetik
/
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

11,431 Views

•

06:56 min

•

March 31, 2017

DOI:

10.3791/55463-v

DOI:
10.3791/55463-v

•

06:56 min
March 31, 2017

•

3 Views
, , , , ,
1Department of Plant and Microbial Biology,University of California Berkeley, 2John Innes Centre,Norwich Research Park, 3Department of Plant and Microbial Biology,University of Zürich, 4USDA-ARS Sunflower and Plant Biology Research Unit

Summary

Automatically generated

Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.

Explore More Videos

DNA ExtractionGenomic DNAPowdery MildewLong-read SequencingConidiaLysis BufferSarkosylChloroform Isoamyl AlcoholIsopropanolEthanolTE BufferRNase

Read Article

Cite this Article

Feehan, J. M., Scheibel, K. E., Bourras, S., Underwood, W., Keller, B., Somerville, S. C. Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing. J. Vis. Exp. (121), e55463, doi:10.3791/55463 (2017).

Download .ris file

Copy

Share Video

.

Copy