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Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models
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Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models

11,137 Views

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10:46 min

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May 03, 2017

DOI:

10.3791/55418-v

DOI:
10.3791/55418-v

•

10:46 min
May 03, 2017

•

5 Views
, , , , ,
1Department of Psychology,West Virginia University, 2Department of Drug Discovery & Development,Auburn University, 3Department of Neuroscience,University of Kentucky Medical Center

Summary

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Here, we describe the setup, software navigation, and data analysis for a spatially and temporally precise method of measuring tonic and phasic extracellular glutamate changes in vivo using enzyme-linked microelectrode arrays (MEA).

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Enzyme-based BiosensorsTonic And Phasic GlutamateAlzheimer’s Mouse ModelsMicroElectrode ArraysNeurotransmitter LevelsNeurotransmitter Release And ClearanceSpatially And Temporally PreciseGlutamate OxidasePBSElectroplatingCalibrationHeating PadPBS Solution

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Hunsberger, H. C., Setti, S. E., Heslin, R. T., Quintero, J. E., Gerhardt, G. A., Reed, M. N. Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models. J. Vis. Exp. (123), e55418, doi:10.3791/55418 (2017).

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