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Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models
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Neuroscienze
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JoVE Journal Neuroscienze
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models

DOI:
10.3791/55418-v

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10:46 min

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May 03, 2017

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, , , , ,
1Department of Psychology,West Virginia University, 2Department of Drug Discovery & Development,Auburn University, 3Department of Neuroscience,University of Kentucky Medical Center

Capitoli

  • 00:05Titolo
  • 00:50Coating the MEA
  • 01:40Electroplating the Electrode
  • 02:46Preparing the Solutions Needed for Calibration
  • 04:48Assemble the Micropipette
  • 05:46General Animal Surgery for MEA Recordings
  • 09:01Results: Extracellular Tonic and Potassium Chloride-evoked Release of Glutamate in Hippocampus
  • 10:18Conclusion

Summary

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Here, we describe the setup, software navigation, and data analysis for a spatially and temporally precise method of measuring tonic and phasic extracellular glutamate changes in vivo using enzyme-linked microelectrode arrays (MEA).

Tags

Enzyme-based BiosensorsTonic And Phasic GlutamateAlzheimer’s Mouse ModelsMicroElectrode ArraysNeurotransmitter LevelsNeurotransmitter Release And ClearanceSpatially And Temporally PreciseGlutamate OxidasePBSElectroplatingCalibrationHeating PadPBS Solution

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