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Measuring Neuromuscular Junction Functionality
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Measuring Neuromuscular Junction Functionality

Measuring Neuromuscular Junction Functionality

17,625 Views

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10:40 min

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August 06, 2017

DOI:

10.3791/55227-v

DOI:
10.3791/55227-v

•

10:40 min
August 06, 2017

•

21 Views
, , , ,
1Department of Mechanical and Aerospace Engineering,Sapienza University of Rome, 2Institute Pasteur Cenci-Bolognetti, DAHFMO-Unit of Histology and Medical Embryology,Sapienza University of Rome, 3Center for Life Nano Science@Sapienza,Istituto Italiano di Tecnologia

Summary

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A functional assessment of the neuromuscular junction (NMJ) can provide essential information on the communication between muscle and nerve. Here we describe a protocol to comprehensively evaluate both the NMJ and muscle functionality using two different muscle-nerve preparations, i.e. soleus-sciatic and diaphragm-phrenic.

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Rizzuto, E., Pisu, S., Nicoletti, C., Del Prete, Z., Musarò, A. Measuring Neuromuscular Junction Functionality. J. Vis. Exp. (126), e55227, doi:10.3791/55227 (2017).

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