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Differenzierung der Primitive und Definitive hämatopoetischen Vorläuferzellen aus menschlichen pluripotente Stammzellen gerichtet
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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Differenzierung der Primitive und Definitive hämatopoetischen Vorläuferzellen aus menschlichen pluripotente Stammzellen gerichtet

10,607 Views

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14:37 min

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November 01, 2017

DOI:

10.3791/55196-v

DOI:
10.3791/55196-v

•

14:37 min
November 01, 2017

•

14 Views
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1Department of Internal Medicine, Hematology Division,Washington University in St. Louis

Summary

Automatically generated

Hier präsentieren wir menschlicher pluripotenter Stammzellen (hPSC) Kultur Protokolle verwendet, um hPSCs in CD34 unterscheiden+ hämatopoetischen Vorläuferzellen. Diese Methode nutzt Bühne-spezifische Manipulation von kanonischer WNT signalisieren Zellen ausschließlich entweder die definitive oder primitive hämatopoetischen Programm angeben.

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Cite this Article

Dege, C., Sturgeon, C. M. Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells. J. Vis. Exp. (129), e55196, doi:10.3791/55196 (2017).

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