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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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発生生物学
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JoVE Journal 発生生物学
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

10,632 Views

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14:37 min

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November 01, 2017

DOI:

10.3791/55196-v

DOI:
10.3791/55196-v

•

14:37 min
November 01, 2017

•

14 Views
,
1Department of Internal Medicine, Hematology Division,Washington University in St. Louis

概要

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Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.

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Human Pluripotent Stem CellsHematopoietic ProgenitorsDirected DifferentiationPrimitive HematopoiesisDefinitive HematopoiesisStage-specific Signal ManipulationEmbryoid BodiesTrypsin EDTAWash BufferHPSC MediaMultigas Incubator

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Cite this Article

Dege, C., Sturgeon, C. M. Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells. J. Vis. Exp. (129), e55196, doi:10.3791/55196 (2017).

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