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对于高度纯化RNA的种子定量转录组分析的隔离中使用的有效方法
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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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对于高度纯化RNA的种子定量转录组分析的隔离中使用的有效方法

9,435 Views

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06:31 min

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January 11, 2017

DOI:

10.3791/55008-v

DOI:
10.3791/55008-v

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06:31 min
January 11, 2017

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3 Views
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1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity,National Institute for Basic Biology, 2Department of Basic Biology,SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology,National Institute for Basic Biology

Tags

RNA ExtractionSeedQuantitative Transcriptome AnalysisArabidopsis ThalianaPVPCell Lysis BufferCentrifugationHomogenizationSpin Column-based MethodHigh-purity RNACereal BiologyGene ProfilesTranscripts

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Kanai, M., Mano, S., Nishimura, M. An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis. J. Vis. Exp. (119), e55008, doi:10.3791/55008 (2017).

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