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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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JoVE Journal 生化学
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

9,448 Views

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06:31 min

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January 11, 2017

DOI:

10.3791/55008-v

DOI:
10.3791/55008-v

•

06:31 min
January 11, 2017

•

3 Views
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1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity,National Institute for Basic Biology, 2Department of Basic Biology,SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology,National Institute for Basic Biology

概要

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We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.

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RNA ExtractionSeedQuantitative Transcriptome AnalysisArabidopsis ThalianaPVPCell Lysis BufferCentrifugationHomogenizationSpin Column-based MethodHigh-purity RNACereal BiologyGene ProfilesTranscripts

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Cite this Article

Kanai, M., Mano, S., Nishimura, M. An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis. J. Vis. Exp. (119), e55008, doi:10.3791/55008 (2017).

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