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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Capitoli
- 00:05Titolo
- 00:55Extraction of Total RNA from Plant Seeds
- 03:05Verification of RNA Quality
- 03:31Reverse Transcription of Total RNA from Seeds
- 04:23Quantitative Real-time PCR Analysis
- 05:01Results: Modified RNA Extraction Process Effectively Removes Inhibitory Compounds
- 06:10Conclusion
We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.
