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ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
Chapters
- 00:05Title
- 00:43Hydrogel Monomer Infusion and Polymerization
- 01:42Electrophoretic Tissue Clearing
- 02:37Immunolabeling of Dense Tissues
- 05:24Results: PRESTO Immunolabeling of ACT-cleared Dense Tissue
- 06:27Conclusion
ACT-PRESTO (active clarity technique–pressure related efficient and stable transfer of macromolecules into organs) enables rapid, efficient, but inexpensive tissue clearing and rapid antibody penetration into cleared tissues by passive diffusion or pressure-assisted delivery. Using this method, a wide range of tissues can be cleared, immunolabeled by multiple antibodies, and volume-imaged.
Tags
ImmunolabelingVolume ImagingNeuronal ConnectionsMolecular CategorizationStructural ChangeSub-cellular AnalysisHydrogelPolymerizationC-PRESTO (centrifugal-assisted Protein Retention Encoding Through Stochastic Trafficking)S-PRESTO (stochastic Protein Retention Encoding Through Stochastic Trafficking)
