This content is Free Access.
ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
11,797 Views
•
07:46 min
•
December 31, 2016
DOI:
Summary
Automatically generated
ACT-PRESTO (active clarity technique–pressure related efficient and stable transfer of macromolecules into organs) enables rapid, efficient, but inexpensive tissue clearing and rapid antibody penetration into cleared tissues by passive diffusion or pressure-assisted delivery. Using this method, a wide range of tissues can be cleared, immunolabeled by multiple antibodies, and volume-imaged.
Explore More Videos
ImmunolabelingVolume ImagingNeuronal ConnectionsMolecular CategorizationStructural ChangeSub-cellular AnalysisHydrogelPolymerizationC-PRESTO (centrifugal-assisted Protein Retention Encoding Through Stochastic Trafficking)S-PRESTO (stochastic Protein Retention Encoding Through Stochastic Trafficking)
Read Article
Cite this Article
Lee, E., Sun, W. ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging. J. Vis. Exp. (118), e54904, doi:10.3791/54904 (2016).
Copy