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方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成
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JoVE 신문 면역학 및 감염병학
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps

方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成

DOI:

10:58 min

March 29, 2017

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Chapters

  • 00:05Title
  • 01:14Isolation of Human Blood-derived Neutrophils by Density Gradient Centrifugation
  • 03:59Isolate the Lipids from the Human Peripheral Blood-derived Neutrophils
  • 05:33High Performance Thin Layer Chromatography (HPTLC) to Semi-quantify the Amount of Lipids
  • 07:53High Performance Liquid Chromatography (HPLC) to Quantify the Amount of Cholesterol
  • 08:48Results: Isolation of Human Blood-derived Neutrophils and Subsequent Lipid Analysis
  • 09:33Conclusion

Summary

자동 번역

脂质是已知在细胞功能中起重要作用。在这里,我们描述了一种方法,以确定嗜中性粒细胞的脂质组合物,重点对胆固醇水平,同时使用HPTLC和HPLC纯化,获得更好的理解嗜中性粒细胞的胞外圈闭形成的基本机制的。

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