/
/
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
9,308 Views
•
10:58 min
•
March 29, 2017
DOI:
1Department of Physiological Chemistry,University of Veterinary Medicine Hannover, 2Fish Disease Research Unit,University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center,Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ),University of Veterinary Medicine Hannover
Summary
Automatically generated
Lipids are known to play an important role in cellular functions. Here, we describe a method to determine the lipid composition of neutrophils, with emphasis on the cholesterol level, by using both HPTLC and HPLC to gain a better understanding of the underlying mechanisms of neutrophil extracellular trap formation.
Read Article
Cite this Article
Brogden, G., Neumann, A., Husein, D. M., Reuner, F., Naim, H. Y., von Köckritz-Blickwede, M. Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps. J. Vis. Exp. (121), e54667, doi:10.3791/54667 (2017).
Copy