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Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
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10:58 min
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March 29, 2017
DOI:
1Department of Physiological Chemistry,University of Veterinary Medicine Hannover, 2Fish Disease Research Unit,University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center,Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ),University of Veterinary Medicine Hannover
Summary
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Lipids are known to play an important role in cellular functions. Here, we describe a method to determine the lipid composition of neutrophils, with emphasis on the cholesterol level, by using both HPTLC and HPLC to gain a better understanding of the underlying mechanisms of neutrophil extracellular trap formation.
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Brogden, G., Neumann, A., Husein, D. M., Reuner, F., Naim, H. Y., von Köckritz-Blickwede, M. Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps. J. Vis. Exp. (121), e54667, doi:10.3791/54667 (2017).
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