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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
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08:17 min
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October 30, 2016
DOI:
1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology,Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy,Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology,Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine,University of Oslo
Summary
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A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell subsets.
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Tognarelli, S., Jacobs, B., Staiger, N., Ullrich, E. Flow Cytometry-based Assay for the Monitoring of NK Cell Functions. J. Vis. Exp. (116), e54615, doi:10.3791/54615 (2016).
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