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Inmunología y contagio
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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

DOI:
10.3791/54615-v

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08:17 min

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October 30, 2016

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, , ,
1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology,Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy,Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology,Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine,University of Oslo

Capítulos

  • 00:05Título
  • 01:01NK Cell Isolation
  • 02:20NK Cell Stimulation
  • 03:52Surface and Intracellular Staining
  • 06:04Results: Representative Healthy Donor NK Cell Stimulation Analysis
  • 07:03Conclusion

Summary

Traducción Automática

Traducción Automática

A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell subsets.

Tags

Flow CytometryNK Cell FunctionsNatural Killer CellsViral InfectionsMalignant DiseasesPediatricsImmunodeficient PatientsNK Cell IsolationEDTA Peripheral Whole BloodNK Cell StimulationK562 Tumor CellsIL-2IL-15

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