Journal
/
생화학
/
Bakterielle Inner-Membran-Display für das Screening einer Bibliothek von Antikörperfragmenten
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
Please note that all translations are automatically generated. Click here for the English version.

Bakterielle Inner-Membran-Display für das Screening einer Bibliothek von Antikörperfragmenten

11,154 Views

•

12:28 min

•

October 15, 2016

DOI:

10.3791/54583-v

DOI:
10.3791/54583-v

•

12:28 min
October 15, 2016

•

3 Views
, , , ,
1Fischell Department of Bioengineering,University of Maryland, 2Department of Chemical and Biomolecular Engineering,University of Maryland

Summary

Automatically generated

We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane.

Explore More Videos

Antibody FragmentInner-membrane DisplayTwin-arginine TranslocationAntibody EngineeringCytoplasmic FoldingSpheroplastsScFv LibraryFractionation BufferLysozymeEDTAMgCl2

Read Article

Cite this Article

Moghaddam-Taaheri, P., Ikonomova, S. P., Gong, Z., Wisniewski, J. Q., Karlsson, A. J. Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments. J. Vis. Exp. (116), e54583, doi:10.3791/54583 (2016).

Download .ris file

Copy

Share Video

.

Copy