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Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
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Bioquímica
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JoVE Revista Bioquímica
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

11,207 Views

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12:28 min

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October 15, 2016

DOI:

10.3791/54583-v

DOI:
10.3791/54583-v

•

12:28 min
October 15, 2016

•

3 Views
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1Fischell Department of Bioengineering,University of Maryland, 2Department of Chemical and Biomolecular Engineering,University of Maryland

Summary

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We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane.

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Moghaddam-Taaheri, P., Ikonomova, S. P., Gong, Z., Wisniewski, J. Q., Karlsson, A. J. Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments. J. Vis. Exp. (116), e54583, doi:10.3791/54583 (2016).

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