ジャーナル
/
/
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
JoVE Journal
生化学
This content is Free Access.
JoVE Journal 生化学
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

DOI:

12:28 min

October 15, 2016

, , , ,

  • 00:05標題
  • 01:11Express scFv Library and Prepare Spheroplasts
  • 03:46Immobilize Target Antigen onto Magnetic Beads
  • 05:05Screen scFv Library
  • 06:32Identify Clones in Secondary Screen Using ELISA
  • 09:51Results: scFv Antibody Library Screening Using the E. coli Twin-arginine Translocation Pathway
  • 11:39Conclusion

概要

自動翻訳

We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane.

関連ビデオ

Read Article