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HIV-1 감염 라이브 차 인간 대 식세포에서 측정 Phagosome 마이그레이션 및 속도
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
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HIV-1 감염 라이브 차 인간 대 식세포에서 측정 Phagosome 마이그레이션 및 속도

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07:32 min

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September 05, 2016

DOI:

10.3791/54568-v

DOI:
10.3791/54568-v

•

07:32 min
September 05, 2016

•

2 Views
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1Inserm U1016, Institut Cochin, CNRS UMR 8104, Université Paris Descartes, Sorbonne Paris Cité

Summary

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We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.

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Cite this Article

Lê-Bury, G., Deschamps, C., Dumas, A., Niedergang, F. Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1. J. Vis. Exp. (115), e54568, doi:10.3791/54568 (2016).

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