/
/
Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
8,066 Views
•
07:32 min
•
September 05, 2016
DOI:
Summary
Automatically generated
We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.
Read Article
Cite this Article
Lê-Bury, G., Deschamps, C., Dumas, A., Niedergang, F. Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1. J. Vis. Exp. (115), e54568, doi:10.3791/54568 (2016).
Copy