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Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
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Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
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September 05, 2016
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We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.
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Lê-Bury, G., Deschamps, C., Dumas, A., Niedergang, F. Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1. J. Vis. Exp. (115), e54568, doi:10.3791/54568 (2016).
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