Journal
/
면역학 및 감염병학
/
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

12,698 Views

•

10:16 min

•

September 15, 2016

DOI:

10.3791/54543-v

DOI:
10.3791/54543-v

•

10:16 min
September 15, 2016

•

4 Views
, , , , , , ,
1Multi-Organ Transplant Program,University Health Network, 2Institute of Medical Science,University of Toronto, 3Divison of Neurosurgery,University Health Network, 4Division of Cardiac Surgery,University Health Network

Summary

Automatically generated

We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.

Explore More Videos

AutoantibodiesAntigen MicroarraysIgGIgMAutoimmunityMultiplexMicroarray PrintingMicroarrayerAntigen DilutionPositive ControlsNegative ControlsSlide PrintingHumidity ControlParallel ScreeningMicrolitersMicrograms

Read Article

Cite this Article

Chruscinski, A., Huang, F. Y. Y., Ulndreaj, A., Chua, C., Fehlings, M., Rao, V., Ross, H. J., Levy, G. A. Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies. J. Vis. Exp. (115), e54543, doi:10.3791/54543 (2016).

Download .ris file

Copy

Share Video

.

Copy