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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

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10:16 min

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September 15, 2016

DOI:

10.3791/54543-v

DOI:
10.3791/54543-v

•

10:16 min
September 15, 2016

•

4 Views
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1Multi-Organ Transplant Program,University Health Network, 2Institute of Medical Science,University of Toronto, 3Divison of Neurosurgery,University Health Network, 4Division of Cardiac Surgery,University Health Network

Summary

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We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.

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Chruscinski, A., Huang, F. Y. Y., Ulndreaj, A., Chua, C., Fehlings, M., Rao, V., Ross, H. J., Levy, G. A. Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies. J. Vis. Exp. (115), e54543, doi:10.3791/54543 (2016).

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