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Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Chapters
- 00:05Title
- 00:46Protoplast Preparation
- 04:08Transformation of U. maydis
- 06:35Verification of Deletion Events: Preparing gDNA
- 09:17Results: Deletion Mutant Experiment
- 10:43Conclusion
We describe a robust gene replacement strategy to genetically manipulate the smut fungus Ustilago maydis. This protocol explains how to generate deletion mutants to investigate infection phenotypes. It can be extended to modify genes in any desired way, e.g., by adding a sequence encoding a fluorescent protein tag.
