Dergi
/
Genetik
/
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
JoVE Journal
Genetik
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Genetik
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

14,029 Views

•

11:42 min

•

September 30, 2016

DOI:

10.3791/54522-v

DOI:
10.3791/54522-v

•

11:42 min
September 30, 2016

•

10 Views
, , , , ,
1Institute for Microbiology,Heinrich-Heine University Düsseldorf, 2Bioeconomy Science Center (BioSC), 3Department of Genetics, Institute of Applied Biosciences,Karlsruhe Institute of Technology, 4Cluster of Excellence in Plant Sciences (CEPLAS),Heinrich-Heine University Düsseldorf

Özet

Automatically generated

We describe a robust gene replacement strategy to genetically manipulate the smut fungus Ustilago maydis. This protocol explains how to generate deletion mutants to investigate infection phenotypes. It can be extended to modify genes in any desired way, e.g., by adding a sequence encoding a fluorescent protein tag.

Explore More Videos

Genetic ManipulationUstilago MaydisFungal BiologyPlant-microbe InteractionsGene DeletionPromoter ExchangeFusion ConstructsProtoplastingTransformationCell CultureOptical DensityContaminationCentrifugationProtoplasting SolutionMicroscopy

Read Article

Cite this Article

Bösch, K., Frantzeskakis, L., Vraneš, M., Kämper, J., Schipper, K., Göhre, V. Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions. J. Vis. Exp. (115), e54522, doi:10.3791/54522 (2016).

Download .ris file

Copy

Share Video

.

Copy