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Biochemistry

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JoVE Journal Biochemistry
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
 

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Article DOI: 10.3791/54466-v • 07:26 min • October 15th, 2016
October 15th, 2016 •
1, 1
1Department of Neuroscience, University of Wisconsin-Madison

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PI(4,5)P2 regulates various cellular functions, but its nanoscale organization in the cell plasma membrane is poorly understood. By labeling PI(4,5)P2 with a dual-color fluorescent probe fused to the Pleckstrin Homology domain, we describe a novel approach to study the PI(4,5)P2 spatial distribution in the plasma membrane at the nanometer scale.

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Single-molecule Super-resolution Imaging Phosphatidylinositol 4,5-bisphosphate Plasma Membrane Fluorescent Probes Nanoscale Distribution Phosphoinositide Signaling Spatial Resolution Detergent Treatment Phospholipid Morphological Studies Plasma Membrane Sheet Preparation Phosphoinositide Labeling Single Molecule Fluorescence Imaging Localization Microscopy Imaging Reconstruction
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