Journal
/
생화학
/
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

13,131 Views

•

10:21 min

•

November 16, 2016

DOI:

10.3791/54458-v

DOI:
10.3791/54458-v

•

10:21 min
November 16, 2016

•

12 Views
, ,
1Center for Bio/Molecular Science & Engineering,Naval Research Laboratory, 2Department of Emergency Medicine,Indiana University of School of Medicine

Summary

Automatically generated

A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is presented.

Explore More Videos

Directed Protein PackagingOuter Membrane VesiclesEscherichia ColiEnzyme PurificationCell-free CatalysisOrganophosphateEnzyme StabilityTherapeutic DevelopmentBioremediationCell-free Catalytic SystemsOMV PurificationUltracentrifugation

Read Article

Cite this Article

Alves, N. J., Turner, K. B., Walper, S. A. Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification. J. Vis. Exp. (117), e54458, doi:10.3791/54458 (2016).

Download .ris file

Copy

Share Video

.

Copy