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Biochemistry

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JoVE Journal Biochemistry
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli
 

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Article DOI: 10.3791/54458-v • 10:21 min • November 16th, 2016
November 16th, 2016 •
1,2, 1, 1
1Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, 2Department of Emergency Medicine, Indiana University of School of Medicine

Capitoli

Riepilogo
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A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is presented.

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Protein Packaging Outer Membrane Vesicles Escherichia Coli Design Production Purification Enzyme-filled Vesicles In Vitro Catalytic Reagents Enzyme Isolation Technique Enzyme Stability Organophosphate Compound Directed Packaging Enzyme Activity Therapeutics Development Remediation Cell-free Catalytic Systems OMV Purification Microbial Cell Cultures
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