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Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
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Biochimica
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JoVE Journal Biochimica
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

13,131 Views

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10:21 min

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November 16, 2016

DOI:

10.3791/54458-v

DOI:
10.3791/54458-v

•

10:21 min
November 16, 2016

•

12 Views
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1Center for Bio/Molecular Science & Engineering,Naval Research Laboratory, 2Department of Emergency Medicine,Indiana University of School of Medicine

Summary

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A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is presented.

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Directed Protein PackagingOuter Membrane VesiclesEscherichia ColiEnzyme PurificationCell-free CatalysisOrganophosphateEnzyme StabilityTherapeutic DevelopmentBioremediationCell-free Catalytic SystemsOMV PurificationUltracentrifugation

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Alves, N. J., Turner, K. B., Walper, S. A. Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification. J. Vis. Exp. (117), e54458, doi:10.3791/54458 (2016).

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