JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
7,035 Views
•
11:00 min
•
December 16, 2016
DOI:
1Department of Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, 2The Graduate School of Biomedical Science,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, 4Center for Neuroscience and Cell Biology,University of Coimbra, 5Department of Pharmacology and Systems Therapeutics,Icahn School of Medicine at Mount Sinai
Summary
Automatically generated
The protocol described here details the induction of a hemogenic program in mouse embryonic fibroblasts via overexpression of a minimal set of transcription factors. This technology may be translated to the human system to provide platforms for future study of hematopoiesis, hematologic disease, and hematopoietic stem cell transplant.
Read Article
Cite this Article
Daniel, M. G., Pereira, C., Bernitz, J. M., Lemischka, I. R., Moore, K. Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program. J. Vis. Exp. (118), e54372, doi:10.3791/54372 (2016).
Copy