需要订阅 JoVE 才能查看此. 登录或开始免费试用。
1Department of Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, 2The Graduate School of Biomedical Science,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, 4Center for Neuroscience and Cell Biology,University of Coimbra, 5Department of Pharmacology and Systems Therapeutics,Icahn School of Medicine at Mount Sinai
Chapters
- 00:05Title
- 00:54Mouse Embryonic Fibroblast Reprogramming
- 04:02Placental Aggregation and Colony Forming Unit Assays in Methylcellulose
- 08:32Results: Transcription Factor-induced Hemogenic Programming of Mouse Embryonic Fibroblasts
- 09:58Conclusion
The protocol described here details the induction of a hemogenic program in mouse embryonic fibroblasts via overexpression of a minimal set of transcription factors. This technology may be translated to the human system to provide platforms for future study of hematopoiesis, hematologic disease, and hematopoietic stem cell transplant.
