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RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing
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RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing

RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing

DOI:
10.3791/54280-v

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08:34 min

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September 27, 2016

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, , , , ,
1Department of Ophthalmology,Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine,Asan Medical Center

Chapters

  • 00:05Title
  • 01:10siRNA Transfection Assay
  • 02:30Western Blot Assay for siRNA-transfected Cells
  • 04:39Evaluation of Cell Migration with Scratch-induced Directional Wounding Assay
  • 05:55Results: Western Blot Analysis and Scratch-induced Directional Wounding Assay to Evaluate Cell Migration
  • 07:21Conclusion

Summary

자동 번역

자동 번역

Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.

Tags

RNA InterferenceHSP27Corneal Epithelial Wound HealingSiRNATransfectionWestern BlotCell CultureProtein ExtractionProtein Quantification

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