JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
basado en la interferencia de ARN Investigación de la Función de la proteína de choque térmico 27 durante la curación de la herida corneal epitelial
7,348 Views
•
08:34 min
•
September 27, 2016
DOI:
Summary
Automatically generated
Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.
Read Article
Cite this Article
Yoo, A., Park, H., Kang, S., Kim, E., Tchah, H., Kim, J. Y. RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing. J. Vis. Exp. (115), e54280, doi:10.3791/54280 (2016).
Copy