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Applicando Fluorescence Resonance Energy Transfer (FRET) per esaminare Effector Translocation efficienza da<em> Burnetii Coxiella</em> Durante siRNA Silencing
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Chapters
- 00:05Title
- 01:02Reverse Transfection of siRNA and Seeding of HeLa 229 Cells
- 04:25Infection of Reverse Transfected Cells
- 05:39Addition of BlaM Substrate to Determine Level of Translocation
- 08:23Results: FRET Measurements of Coxiella burnetti Effector Translocation Efficiency in siRNA Silenced HeLa Cells
- 09:37Conclusion
