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配备了光学切片与成像定时使用解剖显微镜斑马鱼肾脏发展分析

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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

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配备了光学切片与成像定时使用解剖显微镜斑马鱼肾脏发展分析

DOI:

10.3791/53921-v

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10:05 min

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April 07, 2016

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Birgit Perner, Danny Schnerwitzki, Michael Graf³, Christoph Englert²

¹Molecular Genetics,Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), ²Faculty of Biology and Pharmacy,Friedrich Schiller University of Jena, ³Carl Zeiss Microscopy GmbH

Chapters

00:05Title
01:17Collecting Fertilized Eggs
02:27Microinjecting Developing Embryos
04:21Mounting Developing Embryos for Live Imaging
06:59Compensating for Focal Drift
08:04Results: Normal and Disturbed Kidney Development
09:25Conclusion

Summary

자동 번역

这里介绍的方法允许在斑马鱼胚胎器官发育的时间推移分析，通过使用荧光解剖能够执行光学切片和调整的简单的策略来纠正焦点和平面漂移的显微镜。

Tags

Zebrafish Kidney Development Time-lapse Imaging Dissecting Microscope Optical Sectioning 발생학 Fluorescent Microscopy Affordable Technique Model Organisms Transgenic GFP Embryo Micro-injection Morpholino Micro-manipulator

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