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配备了光学切片与成像定时使用解剖显微镜斑马鱼肾脏发展分析

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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

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配备了光学切片与成像定时使用解剖显微镜斑马鱼肾脏发展分析

10,320 Views

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10:05 min

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April 07, 2016

DOI:

10.3791/53921-v

DOI:

10.3791/53921-v

•

10:05 min

April 07, 2016

•

2 Views

Birgit Perner¹, Danny Schnerwitzki¹, Michael Graf^1,3, Christoph Englert^1,2

¹Molecular Genetics,Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), ²Faculty of Biology and Pharmacy,Friedrich Schiller University of Jena, ³Carl Zeiss Microscopy GmbH

Summary

这里介绍的方法允许在斑马鱼胚胎器官发育的时间推移分析，通过使用荧光解剖能够执行光学切片和调整的简单的策略来纠正焦点和平面漂移的显微镜。

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