The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.
Perner, B., Schnerwitzki, D., Graf, M., Englert, C. Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning. J. Vis. Exp. (110), e53921, doi:10.3791/53921 (2016).