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发育生物学
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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

10,362 Views

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10:05 min

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April 07, 2016

DOI:

10.3791/53921-v

DOI:
10.3791/53921-v

•

10:05 min
April 07, 2016

•

2 Views
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1Molecular Genetics,Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), 2Faculty of Biology and Pharmacy,Friedrich Schiller University of Jena, 3Carl Zeiss Microscopy GmbH

Summary

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The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.

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ZebrafishKidney DevelopmentTime-lapse ImagingDissecting MicroscopeOptical Sectioning发育生物学Fluorescent MicroscopyAffordable TechniqueModel OrganismsTransgenic GFPEmbryoMicro-injectionMorpholinoMicro-manipulator

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Perner, B., Schnerwitzki, D., Graf, M., Englert, C. Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning. J. Vis. Exp. (110), e53921, doi:10.3791/53921 (2016).

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