Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Birgit Perner; Danny Schnerwitzki; Michael Graf; Christoph Englert

doi:10.3791/53921

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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

10,359 Views

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10:05 min

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April 07, 2016

DOI:

10.3791/53921-v

DOI:

10.3791/53921-v

•

10:05 min

April 07, 2016

•

2 Views

Birgit Perner¹, Danny Schnerwitzki¹, Michael Graf^1,3, Christoph Englert^1,2

¹Molecular Genetics,Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), ²Faculty of Biology and Pharmacy,Friedrich Schiller University of Jena, ³Carl Zeiss Microscopy GmbH

概要

The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.

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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

概要

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