Journal
/
생체공학
/
Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

DOI:
10.3791/53802-v

•

11:06 min

•

March 01, 2016

•

, ,
1Department of Chemistry & Biochemistry,Fairfield University

Chapters

  • 00:05Title
  • 01:02Make Cyt. c Solutions, Synthesize Silica (SiO2) Sol and Prepare Gel Molds
  • 03:28Prepare Cyt. c-silica Sol-gels
  • 06:10Supercritically Dry Cyt. c-silica Sol-gels
  • 09:46Results: Cyt. c-silica Aerogel Samples and Spectroscopy
  • 10:30Conclusion

Summary

자동 번역

자동 번역

This procedure describes how to encapsulate cytochrome c (cyt. c) in silica (SiO2) sol-gels, process these gels to form bioaerogels, and use these bioaerogels to rapidly recognize nitric oxide (NO) through a gas-phase reaction. This type of protocol may aid in the future development of biosensors or other bioanalytical devices.

Tags

Cytochrome CSilica AerogelBiosensorBioactivityGas-phase ReactionEncapsulationSol-gelPorous MatrixProtein EncapsulationAnalytical DeviceTetraethoxysilaneMethanolWaterAmmonium HydroxidePolypropylene Vials

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Viral Nanoparticles for In vivo Tumor Imaging

Harmonic Nanoparticles for Regenerative Research

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles

Read Article