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Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

DOI:
10.3791/53802-v

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11:06 min

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March 01, 2016

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, ,
1Department of Chemistry & Biochemistry,Fairfield University

Chapters

  • 00:05Title
  • 01:02Make Cyt. c Solutions, Synthesize Silica (SiO2) Sol and Prepare Gel Molds
  • 03:28Prepare Cyt. c-silica Sol-gels
  • 06:10Supercritically Dry Cyt. c-silica Sol-gels
  • 09:46Results: Cyt. c-silica Aerogel Samples and Spectroscopy
  • 10:30Conclusion

Summary

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This procedure describes how to encapsulate cytochrome c (cyt. c) in silica (SiO2) sol-gels, process these gels to form bioaerogels, and use these bioaerogels to rapidly recognize nitric oxide (NO) through a gas-phase reaction. This type of protocol may aid in the future development of biosensors or other bioanalytical devices.

Tags

Cytochrome CSilica AerogelBiosensorBioactivityGas-phase ReactionEncapsulationSol-gelPorous MatrixProtein EncapsulationAnalytical DeviceTetraethoxysilaneMethanolWaterAmmonium HydroxidePolypropylene Vials

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