Journal
/
발생학
/
El seguimiento de las células en el pez cebra transgénico GFP-Uso del sistema de fotoconvertible PSmOrange
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
Please note that all translations are automatically generated. Click here for the English version.

El seguimiento de las células en el pez cebra transgénico GFP-Uso del sistema de fotoconvertible PSmOrange

8,458 Views

•

07:59 min

•

February 05, 2016

DOI:

10.3791/53604-v

DOI:
10.3791/53604-v

•

07:59 min
February 05, 2016

•

5 Views
, , ,
1Medical Faculty Mannheim, Department of Cell and Molecular Biology,Heidelberg University, 2COS and Nikon Imaging Center at the University of Heidelberg,Heidelberg University, 3Excellenzcluster CellNetworks,University of Heidelberg

Summary

Automatically generated

We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.

Explore More Videos

PSmOrangePhotoconversionGFPZebrafishCell TrackingConfocal Microscopy발생학RegenerationTumor ProgressionInvasion488 Nm Laser561 Nm LaserZ-stackRegion Of InterestPhotoactivation BleachingAcquisition StimulationPhotoconversion Protocol

Read Article

Cite this Article

Beretta, C. A., Dross, N., Engel, U., Carl, M. Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System. J. Vis. Exp. (108), e53604, doi:10.3791/53604 (2016).

Download .ris file

Copy

Share Video

.

Copy