Rivista
/
Biologia dello sviluppo
/
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

8,458 Views

•

07:59 min

•

February 05, 2016

DOI:

10.3791/53604-v

DOI:
10.3791/53604-v

•

07:59 min
February 05, 2016

•

5 Views
, , ,
1Medical Faculty Mannheim, Department of Cell and Molecular Biology,Heidelberg University, 2COS and Nikon Imaging Center at the University of Heidelberg,Heidelberg University, 3Excellenzcluster CellNetworks,University of Heidelberg

Summary

Automatically generated

We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.

Explore More Videos

PSmOrangePhotoconversionGFPZebrafishCell TrackingConfocal MicroscopyBiologia dello sviluppoRegenerationTumor ProgressionInvasion488 Nm Laser561 Nm LaserZ-stackRegion Of InterestPhotoactivation BleachingAcquisition StimulationPhotoconversion Protocol

Read Article

Cite this Article

Beretta, C. A., Dross, N., Engel, U., Carl, M. Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System. J. Vis. Exp. (108), e53604, doi:10.3791/53604 (2016).

Download .ris file

Copy

Share Video

.

Copy