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Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

8,458 Views

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07:59 min

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February 05, 2016

DOI:

10.3791/53604-v

DOI:
10.3791/53604-v

•

07:59 min
February 05, 2016

•

5 Views
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1Medical Faculty Mannheim, Department of Cell and Molecular Biology,Heidelberg University, 2COS and Nikon Imaging Center at the University of Heidelberg,Heidelberg University, 3Excellenzcluster CellNetworks,University of Heidelberg

Summary

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We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.

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Beretta, C. A., Dross, N., Engel, U., Carl, M. Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System. J. Vis. Exp. (108), e53604, doi:10.3791/53604 (2016).

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