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Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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May 21, 2015
DOI:
1Department of Obstetrics & Gynecology,Wayne State University School of Medicine, 2School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute,The University of Adelaide, 3Department of Immunology & Microbiology,Wayne State University School of Medicine, 4Perinatology Research Branch,NICHD/NIH/DHHS
Summary
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Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
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Arenas-Hernandez, M., Sanchez-Rodriguez, E. N., Mial, T. N., Robertson, S. A., Gomez-Lopez, N. Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface. J. Vis. Exp. (99), e52866, doi:10.3791/52866 (2015).
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