/
/
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
16,814 Views
•
07:51 min
•
May 21, 2015
DOI:
1Department of Obstetrics & Gynecology,Wayne State University School of Medicine, 2School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute,The University of Adelaide, 3Department of Immunology & Microbiology,Wayne State University School of Medicine, 4Perinatology Research Branch,NICHD/NIH/DHHS
Summary
Automatically generated
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
Read Article
Cite this Article
Arenas-Hernandez, M., Sanchez-Rodriguez, E. N., Mial, T. N., Robertson, S. A., Gomez-Lopez, N. Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface. J. Vis. Exp. (99), e52866, doi:10.3791/52866 (2015).
Copy