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In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
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In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
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May 21, 2015
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Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.
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In Situ DetectionBacteriaDigoxin-labeled DNA Probe16S RRNAPeriodontitisGingival BiopsiesElectron MicroscopyImmunohistochemistryImmunofluorescenceFluorescent In Situ Hybridization (FISH)Species-specificUniversalBacterial FormsEpitheliaInflammatory InfiltratesBlood VesselsPeriodontitisCancerInflammatory Immune Diseases
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Choi, Y. S., Kim, Y. C., Baek, K. J., Choi, Y. In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA. J. Vis. Exp. (99), e52836, doi:10.3791/52836 (2015).
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