/
/
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
10,805 Views
•
11:15 min
•
May 21, 2015
DOI:
Summary
Automatically generated
Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.
Explore More Videos
In Situ DetectionBacteriaDigoxin-labeled DNA Probe16S RRNAPeriodontitisGingival BiopsiesElectron MicroscopyImmunohistochemistryImmunofluorescenceFluorescent In Situ Hybridization (FISH)Species-specificUniversalBacterial FormsEpitheliaInflammatory InfiltratesBlood VesselsPeriodontitisCancerInflammatory Immune Diseases
Read Article
Cite this Article
Choi, Y. S., Kim, Y. C., Baek, K. J., Choi, Y. In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA. J. Vis. Exp. (99), e52836, doi:10.3791/52836 (2015).
Copy