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Medição do isométrica máxima força gerada pelo permeabilizadas fibras musculares esqueléticas
1Department of Orthopaedic Surgery,University of Michigan Medical School, 2Department of Molecular & Integrative Physiology,University of Michigan Medical School, 3Department of Biomedical Engineering,University of Michigan Medical School, 4Department of Surgery, Section of Plastic Surgery,University of Michigan Medical School
Chapters
- 00:05Title
- 01:29Pre-sample Preparation
- 02:25Skeletal Muscle Bundle Dissection, Permeabilization, and Storage
- 04:48Preparation for Single Fiber Testing
- 07:41Single Fiber Testing
- 09:51Results: Activation Rate of Slow and Fast Fibers During Stimulation
- 10:56Conclusion
Análise das propriedades contráteis das fibras musculares esqueléticas, pele quimicamente, ou permeabilizadas oferece um poderoso meio para avaliar a função muscular ao nível da célula muscular único. Neste artigo vamos descrever uma técnica válida e confiável para preparar e testar permeabilizadas fibras musculares esqueléticas in vitro.
