/
/
Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers
This content is Free Access.
24,768 Views
•
11:30 min
•
June 16, 2015
DOI:
1Department of Orthopaedic Surgery,University of Michigan Medical School, 2Department of Molecular & Integrative Physiology,University of Michigan Medical School, 3Department of Biomedical Engineering,University of Michigan Medical School, 4Department of Surgery, Section of Plastic Surgery,University of Michigan Medical School
Summary
Automatically generated
Analysis of the contractile properties of chemically skinned, or permeabilized, skeletal muscle fibers offers a powerful means by which to assess muscle function at the level of the single muscle cell. In this article we outline a valid and reliable technique to prepare and test permeabilized skeletal muscle fibers in vitro.
Read Article
Cite this Article
Roche, S. M., Gumucio, J. P., Brooks, S. V., Mendias, C. L., Claflin, D. R. Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers. J. Vis. Exp. (100), e52695, doi:10.3791/52695 (2015).
Copy